Yeast two-hybrid assays Yeast transformation, mating and cDNA library preparation were performed according to the Matchmaker™ Gold Yeast Two-Hybrid System User Manual (Clontech) For the initial screen, a pGBKT7+ bait was transformed into Y2H Gold (Clontech) and . A yeast library screen was performed using GhHOX3-pDEST32 according to the manufacturer's manual (Invitrogen). Yeast one-hybrid, two-hybrid and three-hybrid analyses were performed using the Matchmaker GAL4 Two-Hybrid System according to the manufacturer's manual (Clontech). Matchmaker Gold Yeast Two-Hybrid System User Manual. Table of Contents. 《Yeast protocol handbook》和《MATCHMAKER GAL4 Two-Hybrid System 3 Libraries User Manual 利用酵母 双杂交 CLONTECH MATCHMARKER SYSTEM 3.
A yeast library screen was performed using GhHOX3-pDEST32 according to the manufacturer's manual (Invitrogen). Yeast one-hybrid, two-hybrid and three-hybrid analyses were performed using the Matchmaker GAL4 Two-Hybrid System according to the manufacturer's manual (Clontech). The Matchmaker Gold Yeast Two-Hybrid System is the only yeast two-hybrid system with four genuine reporters and three different bait recognition sequences. Background growth is not possible due to the potency and stability of Aureobasidin A, a novel yeast antibiotic that kills all non-resistant cells. Matchmaker Gold Yeast Two-Hybrid System User Manual () www.doorway.ru Takara Bio USA, Inc. Page 4 of 41 I. Introduction Protocol Overview Principle of the Two-Hybrid Assay In a Matchmaker GAL4-based two-hybrid assay, a bait protein is expressed as a fusion to the Gal4 DNA -binding domain (DNA-BD), while libraries of prey.
Matchmaker Gold Yeast Two Hybrid System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/, based on 1 PubMed citations. 22 Constructing and screening Matchmaker One-Hybrid and Two-Hybrid libraries. 6. Figure 5. Reporter gene constructs in yeast strains AH and. J Biotechnol –; Clontech () Matchmaker™ GAL4 two-hybrid system 3 libraries user manual. www.doorway.ru
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